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Title Antioxidant and Antimutagenic Activities of Syzygium antisepticum (Blume) Merr.& L.M. Perry Leaf Extracts
Authors Methin Phadungkit, Surapong Suksiripattanapong, Kreepol Manwiwattanakun
Abstract

       Syzygium antisepticum (Blume) Merr.& L.M. Perry (Myrtaceae) is used in Thai traditional cousin as a local vegetable. Leaves of the plant are used as an antiflatulence in Thai traditional medicine. The objectives of this study were to test the antioxidant activity and the antimutagenic activity. The leaf extracts in various solvents were prepared by the maceration method in ethanol. The solvent was evaporated to dryness by a rotary evaporator to obtain the crude extract. The crude extract was partitioned between purified water and hexane. The water portion was then partitioned with ethyl acetate. The organic solvents were evaporated to dryness by a rotary evaporator to obtain the hexane and the ethyl acetate fractions. The water extract was prepared by boiling with purified water and dried by using lyophilization. The antioxidant activity was tested by the DPPH assay. The antimutagenic activity was determined by the Ames test in Salmonella typhimurium TA 98 and TA 100 strains. The results showed that the ethyl acetate fraction, the crude extract and the water extract exhibited strong DPPH scavenging activities with IC50 value ranged from 17.28 ± 0.12 - 15.96 ± 0.16 μg/mL. All of herbal extracts at the concentration of 200 μg/plate exhibited strong antimutagenic activity both in TA 98 and TA 100 strains. The present study suggested that S. antisepticum extracts have high potential to develop as functional foods for prevention of diseases caused by free radicals or mutation.
Keywords Syzygium antisepticum (Blume) Merr.& L.M. Perry, Antioxidant, Antimutagenic
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